Analysis Note

Positive ControlHeLa cells

Application

Immunoblotting (1:1000)r>Immunoprecipitation (see comments)

General description

Recognizes the ~100 kDa 19S proteasome S2-subunit in HeLa cells and partially purified 26S proteasome preparations. Also recognizes an ~50 kDa potential subunit degradation product.

Immunogen

Human

recombinant, human 19S proteasome S2-subunit

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Also detects an ~50 kDa band, presumably corresponding to a subunit degradation product. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Kloetzel, P.M. 2001. Nat. Rev. Mol. Cell Biol.2, 179.Yew, P.R. 2001. J. Cell Physiol.187, 1.Chaturvedi, K., et al. 2000. Biopolymers55, 334.Orlowski, M., and Wilk, S. 2000. Arch. Biochem. Biophys.383, 1.Pickart, C.M. 2000. Trends Biochem Sci.25, 544.

Packaging

100 µL in Glass bottle

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 1 mg/ml BSA.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)